This article reviews the development and progress related to lactic acid separation and recovery from fermentation broth. However, the recovery and separation of lactic acid from microbial fermentation media are relatively complicated and expensive, especially in the process relating to second-generation (2G) lactic acid recovery. Fermentation has been considered the preferred method for producing high-purity lactic acid in the industry over chemical synthesis. Its global market demand has significantly increased in recent years, with a CAGR of 18.7% from 2019 to 2025.
#LIQUID LIQUID EXTRACTION IN DOWNSTREAM PROCESSING HOW TO#
Read more about how to correctly acknowledge RSC content.Lactic acid has become one of the most important chemical substances used in various sectors. Permission is not required) please go to the Copyright If you want to reproduce the wholeĪrticle in a third-party commercial publication (excluding your thesis/dissertation for which If you are the author of this article, you do not need to request permission to reproduce figuresĪnd diagrams provided correct acknowledgement is given. Provided correct acknowledgement is given. If you are an author contributing to an RSC publication, you do not need to request permission Please go to the Copyright Clearance Center request page. To request permission to reproduce material from this article in a commercial publication, Provided that the correct acknowledgement is given and it is not used for commercial purposes. This article in other publications, without requesting further permission from the RSC, pluvialis production.ĭirect extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid–liquid chromatographyĬreative Commons Attribution-NonCommercial 3.0 Unported Licence. This allows direct astaxanthin extraction at the site of H. The mechanical cell disruption, drying and extraction with supercritical CO 2 in the conventional astaxanthin production can be replaced by a direct astaxanthin extraction process, using a liquid–liquid chromatographic column. Using a liquid–liquid chromatographic column, astaxanthin concentrations reaching 500 mg L −1 were obtained, corresponding to eightfold concentration of the astaxanthin content in the fermentation broth.
It was shown that astaxanthin could be extracted from the zoospores directly from the algal broth using different solvents with ethyl acetate, yields reaching 85% were achieved in a shake-flask extraction.
pluvialis cyst cells was initiated, resulting in the release of flagellated zoospores into the fermentation broth. In this study, an alternative extraction process was established, using a liquid–liquid chromatographic column to directly extract astaxanthin from the fermentation broth into a solvent. During the subsequent downstream processing, the algal broth is harvested by centrifugation, dried and mechanically disrupted finally, astaxanthin is extracted with supercritical CO 2. Astaxanthin synthesis involves the formation of a rigid cell wall that impedes direct astaxanthin extraction into a solvent. pluvialis) is used for biotechnological production of the red carotenoid astaxanthin. The microalgae Haematococcus pluvialis ( H.
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